Thoracic Research and Practice
Original Article

Tocilizumab Reduces Lung Injury in a Rat Lung Ischemia and Reperfusion Model

1.

Bahçelievler State Hospital Anesthesiology and Reanimation Clinic, İstanbul, Turkey

2.

Department of Medical Biochemistry, Health Sciences University İstanbul Training and Research Hospital, İstanbul, Turkey

3.

Department of Cardiovascular Surgery, Koşuyolu High Specialization Education And Research Hospital, İstanbul, Turkey

4.

Department of Casdiovascular Surgery, Bahçelievler State Hospital, İstanbul, Turkey

5.

Department of Pathology, Health Sciences University İstanbul Training and Research Hospital, İstanbul, Turkey

Thorac Res Pract 2024; 25: 62-67
DOI: 10.5152/ThoracResPract.2024.23061
Read: 575 Downloads: 191 Published: 19 February 2024

OBJECTIVE: In this study, the effect of tocilizumab (TCZ) on lung tissue in lung ischemia–reperfusion (I/R) injury in rats was investigated.

MATERIAL AND METHODS: A total of 24 Wistar rats were divided into 4 equal groups, with 6 rats in each group: Left lung I/R was applied to I/R groups. In the I/R groups, the left lung hilum was clamped for 45 minutes, and then the clamp was removed and reperfused for 120 minutes. In the TCZ groups, 4 mg/kg and 8 mg/kg of TCZ were administered intraperitoneally to the rats 30 minutes before surgery.

RESULTS: The tumor necrosis factor-alpha mean value was not statistically significant between the groups (P = .091). Statistically significant results were observed between group I/R-TCZ (8 mg/kg) and group I/R for catalase. (P = .005). Statistically significant results were observed between group I/R-TCZ (8 mg/kg) and group I/R for malondialdehyde. (P = .009). The difference in total ischemia score between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R was statistically significant (P < .001). In terms of alveolar hemorrhage, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .01 and P = .002, respectively). There was a statistically significant difference between group I/R-TCZ (8 mg/kg) and group I/R in terms of neutrophil accumulation (P = .01). In terms of interstitial edema, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .006 and P = .001, respectively). In terms of pulmonary edema, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .01 and P = .009, respectively).

CONCLUSION: Lung tissue may be affected by I/R injury and this damage can be reversed with the use of TCZ.

Cite this article as: Özgür Y, Işık R, Orhan B, et al. Tocilizumab reduces lung injury in a rat lung ischemia and reperfusion model. Thorac Res Pract. 2024;25(2):62-67.

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