Thoracic Research and Practice

T-Cell Changes in Peripheral Blood of Asthmatics During Bronchoconstriction Caused by Histamine Bronchoprovocation


Osmangazi University, Medical Faculty, Departments of Pulmonary Diseases and Internal Medicine, Eskişehir, Turkey


Department of Internal Medicine, Division of Hematology, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey


Department of Chest Disease, Osmangazi University Medical Faculty, Eskişehir, Turkey


Department of Chest Diseases, Kadıköy Florence Nightingale Hospital, İstanbul, Turkey

Thorac Res Pract 2004; 5: Turkish Respiratory Journal 68-72
Read: 351 Downloads: 207 Published: 11 October 2021

To study the role of T lymphocyte subtypes in acute bronchoconst' rictive responses caused by nonspecific stimuli, we performed bronchial histamine challenge tests in 24 stable asthmatic patients and in 10 nonatopic-nonasthmatic controls. None of the asthmatic patients had had an attack or suffered from an upper respiratory tract infection within the 8 weeks before the challenge test, nor had been receiving corticosteroids. Short acting P2 agonists were stop­ped 6 hours and long acting P2 agonists 12 hours before the challen­ge test. T lymphocyte subgroups, T lymphocyte activation markers and natural killer cells were determined in the peripheral blood be­fore and after the histamine challenge test by two color flow cyto­metry. The results showed that while there were no changes in the percentages and absolute numbers of CD3+CD4+, CD16+CD56+, CD5+CD25+ and CD5+HLA-DR+ cells after the challenge test, CD3+CD8+ cells were higher both as percentages [23.9% (16.7- 36.7) versus 24.5% (16.7-40.5), p<0.05] and as absolute numbers [574 (65-1204)/mm3 versus 686 (163-1192)/mm’, p<0.05] after the challenge test in the asthmatics. Control subjects showed no diffe­rences after nonspecific bronchoprovocation. In view of these findings it can be stated that CD8+ T lymphocytes may have a role in the acute bronchoconstrictive response to nons­pecific stimulus in asthmatic patients.

EISSN 2979-9139